Guide to Patagonia's Monsters & Mysterious beings

I have written a book on this intriguing subject which has just been published.
In this blog I will post excerpts and other interesting texts on this fascinating subject.

Austin Whittall

Thursday, December 3, 2015

The Diego blood group and the peopling of America

The Diego Blood group system was discovered by chance in 1953. At that time, a woman who lived in Caracas, Venezuela gave birth to her second child. The newborn became ill with hemolytic jaundice which was caused by an incompatibility between baby and mother's blood. The samples were sent fot analysis and the outcome wwas a new private antigen which was named after the baby, Diego (he died shortly after).

Further studies identified two different variants Dia and Dib. The first was found to be quite frequent among Amerindians and some Asian populations but absent among Caucasians, Africans and Australian Aboriginals. The second variant was common around the world.

Dia was also found among Polish people, and it is conjectured that it reached Europe via the Tartar - Mongol invasions in the 1300s.

The two variants arise from a mutation in the SLC4A1 gene. Where an amino acid change provokes Dia. As it is not found in all Asian populations it may have arisen in a confined area of Asia and moved into America with part of these people when they migrated into the New World.

In America there are two variants Di(a-) common in South America and NW North America while the other one D(a+) is found elsewhere. This may be due to the fact that the Di(a-) people are the remnants of a first wave of migrants, later over ran by a Di(a+) population. This theory was proposed by M. Layrisse and J. Wilbert [1] who wrote:

"The Diego blood group is an exclusive Mongoloid gene marker, although it is not present in all Mongoloid populations. The absence of the gene in Waica Indians and its very low frequencies in Warrau and Yaruro Indians of South America suggest that it represents a genetic characteristic of Marginal American Indians. Since Marginal Indians are considered to be early comers to the New World, we suggest that Diego-negative t populations were the first to arrive and to extend throughout South America, while the Diego-positive tribes came later."

Interestingly this theory would be completed by a later third wave that peopled the Arctic region where the Dia type is absent.

A recent paper by Bégat C, Bailly P, Chiaroni J and Mazieres S [2] gives another explanation:

"...For this purpose we assumed that the geographic distribution of the DI*A allele coding for Dia is not random but rather coincided with cultural traits according to the gene-culture comigration concept.
High frequencies were observed in the Peruvian Andes, on the Guyanese Plateau, in the southeastern Amazonian Basin, in a region comprising the Brazilian state of Pará, and along the Tapajos, Xingu and Araguaia tributaries of the Amazon River. Low frequencies occurred mainly in North America, in Chaco and at southern tip of South America. The DI*A allele was totally absent in the Arctic (Eskimo and Tlingit), Panama Isthmus (Bribri and Teribe), Tierra de Fuego (Alacalufe), and a few pocket areas in North America (Cherokee), Northern Brazil/Southern Venezuela (Ninam and Yanomama), and the Chaco area (Ayoreo, Zamucoan).

They go on to explain that the Ayore suffered from a "severe founder effect", and this may explain the lack of DI*A in them. Then they explain its absence among the Panama Isthmus populatons because these Chibchan-speakers "predecessors have lived in isolation from Central and South Amerindians since the early Holocene" [2].

The Fuegians lack this allele because of "isolation by distance with ensuing drift" [2] and its void in Northern North America is due to a "Secondary migration ... of non-Amerind-speakers, i.e., Eskimo-Aleut and Na-Dene speakers, from Alaska to Greenland and Southwestern North America.", they back-migrated into Siberia too, and that explains the link between their language and the Yeniseian languages in Siberia where the Yeniseian speakers also lack the Di*A allele. Then came even more gene flow carrying the DI*B allelle into the boreal part of the New World.


  • 5% Chinese
  • 12% Japanese
  • 54% South American Natives
  • 4% North India
  • 0.47% Polish
  • 0.01% Other Caucasians

I must admit that the Diego blood Group is an oddity, found in many non-African It has an exclusively Amerindian variant the Dia variant but, and this is interesting: An antibody of the Diego antigen, Anti-Di(a), is the cause of hemolytic transfusion reactions and also of hemolytic disease of the newborn (like happened to the baby, Diego). The presence of this antigen, or Dia alloimmunization has been observed among different Asian and South American Amerindian populations. A study by Baleotti et al., (2014) [3] found that:

"...HLA-DRB1*07:01 allele was overrepresented in Dia-alloimmunized patients compared to nonimmunized patients and to healthy donors.
... Individuals carrying the HLA-DRB1*07:01 allele present an increased risk for Dia alloimmunization...

This means that those with the lowest frequency of this type of HLA, which in the map below would mean those native to the areas shaded in blue or pale blue, would not have the Anti-Di(a) antigen, because they have Di(a). And the map is clear: The Americas and east Asia. But! why is Australia, Melanesia and Africa also blue?

Also the Altai region (i.e. Denisova-land) is blue. Could the Australia -Melanesia - Americas mean some Denisovan introgression? But what about Southern and Western Africa?.

Could this imply some introgression there, in Africa, of an archaic hominin? See my post an ancient Y chromosome lineage, which mentions a possible archaic-human admixture in a Nigerian site known as Iwo Eleru, where human skeletal remains with both archaic and modern features were found and dated to ~13 kya.

The map below shows the global distribution of DRB1*07:01:01:01, (Source).

And this is the image from [2] showing the distribution of the Diego factor in America (black = none, dark blue= high)

I have the impression that Diego factor is archaic, not the result of a bottleneck.

[1] M. Layrisse, J. Wilbert, Science (1961). Absence of the Diego Antigen, a Genetic Characteristic of Early Immigrants to South America, 13 October 1961, Vol. 134 no. 3485 pp. 1077-1078 DOI: 10.1126/science.134.3485.1077
[2] Bégat C, Bailly P, Chiaroni J, Mazieres S (2015) Revisiting the Diego Blood Group System in Amerindians: Evidence for Gene-Culture Comigration. PLoS ONE 10(7): e0132211. doi:10.1371/journal.pone.0132211
[3] Baleotti, W., Ruiz, M. O., Fabron, A., Castilho, L., Giuliatti, S. and Donadi, E. A. (2014), HLA-DRB1*07:01 allele is primarily associated with the Diego a alloimmunization in a Brazilian population. Transfusion, 54: 2468–2476. doi: 10.1111/trf.12652

Patagonian Monsters - Cryptozoology, Myths & legends in Patagonia Copyright 2009-2014 by Austin Whittall © 


  1. So Austin, what you're basically saying is that the Diego blood group is another piece of the jigsaw or piece of evidence for an earlier migration into the Americas. In other words what some recent blog posts have begun to refer to as a'Ghost Population' of barely discernable relict DNA, an echo, if you will of an earlier (say 25-30 kya) wave of migration? Have I read you correctly?
    Oh yeah! That is one seriously mind bending piece of work by the way! How many hours did that take you to work up? Congratulations fir the sheer effort put in!

  2. Thank you Neil!
    The point is that when odd things turn up among Native Americans the answer is: bottleneck - founder effect. So here we have a population unique in many ways, which supposedly arose from a few families stranded in Beringia for thousands of years... and it is from this limited repertoire that all these unique features appeared: hundreds of languages, HLAs, mtDNA haplogroups, Y haplogroups, and more...
    I think that the ghost population reached the New World long ago and that it is them who provided the diversity to H. sapiens latecomers.


Hits since Sept. 2009:
Copyright © 2009-2014 by Austin Victor Whittall.
Todos los derechos reservados por Austin Whittall para esta edición en idioma español y / o inglés. No se permite la reproducción parcial o total, el almacenamiento, el alquiler, la transmisión o la transformación de este libro, en cualquier forma o por cualquier medio, sea electrónico o mecánico, mediante fotocopias, digitalización u otros métodos, sin el permiso previo y escrito del autor, excepto por un periodista, quien puede tomar cortos pasajes para ser usados en un comentario sobre esta obra para ser publicado en una revista o periódico. Su infracción está penada por las leyes 11.723 y 25.446.

All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted in any form or by any means - electronic, mechanical, photocopy, recording, or any other - except for brief quotations in printed reviews, without prior written permission from the author, except for the inclusion of brief quotations in a review.

Please read our Terms and Conditions and Privacy Policy before accessing this blog.

Terms & Conditions | Privacy Policy

Patagonian Monsters -